Bacteriological analysis of the water in the Caño Martín Peña
Bacteriological analysis of the water in El Cao Martn Pea Detection and quantification of Enteric Pathogenic Bacteria Salmonella and Shigella spp. Mario R. Barrera George M. Rivera Paola Tabaro Mentor: Prof. Mayra Roln University of the
Sacred Heart 1 / march/ 2013 Meet the Team George M. Rivera Mario R. Barrera Mentor: Prof: Mayra
Roln Paola Tabaro Meet the Team 2 0 1 2
2 0 1 3 Cao Martn Pea Is an invaluable natural habitat for the Metropolitan area of San Juan.
Cao Martn Pea + During the recurring years the mangroves and lagoons stretched of the Cao have been contaminated and stretched out into smaller measurements. Cao Martn Pea
The deterioration of this area has taken its toll on the residents lifestyles. How to Improve !! The decontamination of the lagoon that boards the community. Rehabilitation of the
estuary system. To broaden the communitys knowledge of the microbial presence and the harms that can be encountered. Our objectives
+ To detect the presence of pathogenic enteric bacteria such as Salmonella and Shigella SPP. + To acquire knowledge of the microbial presence found in the
Cao Martn Pea. + Refine research skills through different bacteriological techniques. + Analyze and compare the degree of contamination found with past investigations.
+ Raise awareness to the community. Hypothesis In the past year our fellow classmates were able to detect the presence of Shigella serotypes A,B and C. The Cao Martn Pea has excessive levels of coliforms, indicators of fecal matter that represent a hazard for the community.
Hypothesis + Because of this information we can say there is a high probability of encountering Salmonella and Shigella SPP. Bacteria due to the high coliform levels resulting from water contamination by fecal matter. Coliform bacteria Rod-shaped Gram
negative bacillus. Lactose fermenters with the production of acid and gas when incubated at 3537C. Easy to culture and their presence is used to
indicate fecal contamination by other pathogenic organisms in the water. Enterobacteriaceae Total coliforms: Escherichia Klebsiella Serratia
Yersinia Black Colony Citrobacter freundi Pale pink Serratia plymuthica Metallic Green
E. coli Types of Bacteria Bacteria Serratia marcenscens Enterobacter aglomerans Enterotubes Shigella Enterotube
Findings Year Quantity Year Quantity 2000
4,000,000 2008 118,000 2001 5,500,000 2009
3,335,000 2002 4,000,000 2010 6,800,000 2003
2,000,000 2011 60,000,000 2004 100,000 2012
69,000,000 2005 73,000 2013 25,333,333 2006
80,000 2007 57,000 Number of bacteria/ ml of water Findings on Dilutions Station #1
1 col in 10 : 10,000,000 bact/ml Station #2 4 col in 10: 4,000,000 bact/ml Station #3
62 col in 10: 62,000,000 bact/ml Findings on Filtration Citrobacter freundi Pure > 100 col (TNTC) Cedecea lapagei Dilute: 5 col * 1000: 5,000 bact/100ml Klebsiella pneumoniae Yersinia enterocolitica
Station #2 Citrobacter freundi Pure>100 col (TNTC) Cedecea lapagei Dilute: 40 col * 1000: 40,000 bact/100ml Enterobacter agglomerans Klebsiella ozaenae Station #3 Pure>100 col (TNTC) Enterobacter Dilute: 3 col * 1000: 3,000 bact/100ml
agglomerans Station#1 Conclusions The high level of contamination is due to: Stagnant water in
some areas. Sewage and water discharge in to the cano. Deposition of debris into the community. Conclusions of the Research From 2004 to 2008 the amount of bacteria were
less than a million/ ml of water. This could be due to the removal of debris Effort of government and people that lives in the community. + During our Research period we noticed a sudden decrease in the level of bacteria.
+ Although we were not able to isolate colonies of Salmonella, we did isolate Citrobacter freundi, Yersinia enterocolitica and Shigella Spp. + Which are indicative of the severity of contamination and a possible source of contamination with pathogens to the residents of nearby areas. We recommend: To maintain the movement of dredging and
cleaning of the entire San Juan Estuary. We saw that according to data, levels of bacteria were down under a million. With a little of effort is possible to maintain to provide a better life to the people. To Help organizations and people that live there in a constant way not only, to show interest when elections are near. References
Http://www.mayoclinic.com/health/shigella/DSOO 719 Http://www.cdc.gov/salmonella/enteritidis/ Centers for Disease Control and Prevention. October 2005. Shigellosis. http://www.cdc.gov/nczved/divisions/dfbmd/diseas es/shigellosis/ Indian and Northern Affairs Canada. May 2003.
First Nations Water Management Strategy. Http://www.ainc-inac.gc.ca/ai/arp/es/0506/fnwms/f nwms-eng.asp References Public Health Agency of Canada:Notifiable Diseases on- line. December 2003. Shigellosis. http://dsol-smed.hc-sc.gc.ca/dsol-smed/ndis/diseases/shi g_e.html www.safewater.com World Health Organization. 2005. Guidelines for the
control of shigellosis, including epidemics due to shigella dysenteriae type 1. http://whqlibdoc.who.int/publications/2005/9241592330. pdf United States Pathogenic Microorganisms and Natural Toxins Handbook. January 2002. Shigella. Http://pdf.usaid.gov/pdf_docs/ PNADO152.pdf
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