Different technologies for producing Algae Continuous ...

Different technologies for producing Algae Continuous ...

Different technologies for producing Algae Batch and continuous production methods Live food aquaculture training course www.aquatrain.org Main culture systems Batch culture 300 or 500 litre sacs

D-ended tanks Continuous culture Connected sac system (SeaSalter) Tubular fence (BioFence) spiral (AddaVita) heterotrophic production (Aqua Fauna) Tubular Live food aquaculture training course

www.aquatrain.org Batch Culture Indoors Greenhouse Live food aquaculture training course www.aquatrain.org Tank culture Advantages - large volumes

Disadvantages - weak culture, prone to contamination Small tanks large tanks Live food aquaculture training course www.aquatrain.org SeaSalter System Continuous Algal Production, Systems, SeaCAPS

Adapted batch culture technique Plastic Sacs connected together dosing pump water and nutrient supply overflow into common collector Live food aquaculture training course www.aquatrain.org SeaSalter SeaCAPS

water supply Live food aquaculture training course www.aquatrain.org SeaSalter SeaCAPS algae collection Live food aquaculture training course www.aquatrain.org SeaSalter SeaCAPS

Equipment required Live food aquaculture training course www.aquatrain.org SeaSalter SeaCAPS Water pasteurised before distribution Nutrients and carbon dioxide are metered in continuously All pipework is designed to be steam sterilised

weekly They can be installed indoors, in greenhouses (with or without supplementary lighting outdoors under shade Live food aquaculture training course www.aquatrain.org SeaSalter SeaCAPS Each

500 litre bag will yield 80-120 litres of algae per day. The expected production of a 40-bag unit would be 4-5 cubic metres per day of quality algae. Cell density is dependent on light availability and required flow rates draws less than 3kW of power excluding lights Live food aquaculture training course www.aquatrain.org SeaSalter SeaCAPS

Requires about 2 man-days per week are enough to run a 40-bag installation and take care of stock and starter cultures 500 Litre polythene bags are run for an average of four months and discarded before collapsing A typical greenhouse-type installation with artificial illumination can attain a cell density of 4000 cells/microlitre. Live food aquaculture training course

www.aquatrain.org SeaSalter SeaCAPS MaxMar Mariscos, Laguna Manuela in Baja California with a 12 bag layflat system to support their hatchery to grow C. gigas and scallops. Live food aquaculture training course www.aquatrain.org SeaSalter SeaCAPS lay flat bag systems outdoors which

operate with no artificial light. Live food aquaculture training course www.aquatrain.org SeaSalter SeaCAPS Norway Scalpro AS produce scallops and oysters at Rong, near Bergen. Their upright system built into an old fish processing plant has been working since 1996 Live food aquaculture training course

www.aquatrain.org Tubular reactors BioFence Addavita Why do we change from batch to continuous high density production? Live food aquaculture training course www.aquatrain.org

Theory In a tank or algal bag system, the zone of penetration is low. Once the cells move away from that surface then light cannot penetrate and photosynthesis ceases. However, this dark area allows more complex protein building to occur within the algae. Due to sacs diameter the ratio of light to dark area is small and so algal growth is limited. Live food aquaculture training course www.aquatrain.org

Transparent tubes, self-cleaning Very tough, very clear food grade plastic tubes. They are self cleaning thanks to patented special cleaning beads that continuously circulate with the algae. This enables the system to be run for extended periods without culture crashes or cleaning. Live food aquaculture training course www.aquatrain.org Space saving and reliable Space saving - The tubes can be mounted vertically in a

fence like arrangement or horizontally on the ground or at an angle on a roof. They take up a lot less room than other growing methods and of course, they are growing far more concentrated algae so even less space is required. Reliable -Can operate for long periods before crashing, consistent quality of product. Efficient - save on labour costs Live food aquaculture training course www.aquatrain.org

Ready prepared nutrients There are two major sources of error in growing algae; preparation and contamination ready made nutrients with complete formulation in sterile, concentrated liquid form Live food aquaculture training course www.aquatrain.org BioFence Clear pipe fence reactor

Live food aquaculture training course www.aquatrain.org BioFence Live food aquaculture training course www.aquatrain.org BioFence Production Species Concentrations reached,

cells x 106 / ml BIOFENCE BAG Outdoors* Indoors Nannochloropsis 280

150 20 Chlorella 400 200 20

Isochrysis 40 20 3 Tetraselmis 6

2.5 0.4 Chaetoceros 15 8 3

Live food aquaculture training course www.aquatrain.org BioFence Production TYPICAL BIO-FENCE GROWTH DATA FOR NANNOCHLOROPSIS OCULATA 600Litre Bio-Fence, Polytunnel, natural light, Temperature 22-26C, Salinity 28ppt. Day 1 2 3 4

5 6 7 8 9 10 11 12 13 14 15

Bio-Fence concentration Quantity of algae harvested Million cells per ml Litres 10,6 17,4 31,1 75,4 123,7 200 150,5 200 225,5

200 254 200 280 264 250 264 175 200 220 200 234

200 219 200 217 200 Live food aquaculture training course www.aquatrain.org Comments Innoculation

Full Harvest BioFence Greenhouse Live food aquaculture training course www.aquatrain.org BioFence indoors Live food aquaculture training course www.aquatrain.org

AddaVita Clear pipe spiral reactor Live food aquaculture training course www.aquatrain.org Live food aquaculture training course www.aquatrain.org AddaVita reactor

Live food aquaculture training course www.aquatrain.org AddaVita outdoors and indoors Live food aquaculture training course www.aquatrain.org AddaVita controls Live food aquaculture training course

www.aquatrain.org Heterotrophic production Live food aquaculture training course www.aquatrain.org Boa photobioreactor Live food aquaculture training course www.aquatrain.org

Prism photobioreactor Live food aquaculture training course www.aquatrain.org Tubular photobioreactor Motor Schematic Diagram of Tubular Photobioreactor

Height 1.5m Diameter 30 cm Live food aquaculture training course www.aquatrain.org Tubular photobioreactor

Live food aquaculture training course www.aquatrain.org

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