DNA Fingerprinting - Iowa State University

DNA Fingerprinting Gel Staining and Analysis Student Instructions Gel Staining and Analysis Step 1 When electrophoresis is completed, turn off the power supply. Remove the lid from the

box. Lift the lid to remove it from the box. DNA Fingerprinting Student Instructions Gel Staining and Analysis Step 2 To stain the gel to make the DNA band visible, remove the gel tray from the electrophoresis unit

and place the tray into a plastic container. Slide the gel off the tray by pushing on one end of it. DNA Fingerprinting Student Instructions Gel Staining and Analysis Step 3 Add the Carolina Blu DNA Stain, making sure

the gel is completely immersed. Do not pour the stain directly on the gel. Stain the gel for 15 minutes. Agitate gently, if possible. DNA Fingerprinting Student Instructions Gel Staining and Analysis Step 4

Pour the stain back into the bottle. Cover the gel with distilled water to destain. Agitate gently, if possible, for 30 to 40 minutes, changing the water every 10 minutes. DNA Fingerprinting Student Instructions Gel Staining and Analysis

Step 5 The gel can be displayed to the class by sliding it onto a piece of plexiglass and placing it on a white sheet of paper or on a white-light viewing box. DNA Fingerprinting Student Instructions

Gel Staining and Analysis Step 6 Ideal gel Stained with ethidium bromide and viewed under UV light source DNA Fingerprinting Student Instructions