Environmental Division - Exploring the benefits of a direct ...

Environmental Division - Exploring the benefits of a direct ...

AS/NZS 4187:2014 Water Quality Analysis Zachary Chataway R IG H T S O L U T I O N S | R IGH T PA RT NE R Topics 1. AS/NZS 4817:2014 2. Water Quality Parameters 3. What is TVC? 4. What are Endotoxins?

5. Sampling & Transport 6. Guideline Report 4187 Guideline Presentation- March 2015 2 1. AS/NZS 4817:2014 AS/NZS 4817:2014 - Reprocessing of reusable medical devices in health service organizations.

Replaces AS/NZS 4817:2003 - Cleaning, disinfecting and sterilizing reusable medical and surgical instruments and equipment, and maintenance of associated environments in health care facilities. 4187 Guideline Presentation- March 2015 3

2. Water Quality Parameters Page 55 of AS/NZS 4187:2014 4187 Guideline Presentation- March 2015 4 3. What is TVC? TVC = Total Viable Count HCC, HPC, TCC

Indication of the size of the microbial community in a water system Microbes will flourish with no disinfection measure present ie Chlorine 4187 Guideline Presentation- March 2015 5

3. What is TVC how is it measured? Pour plate 1ml inoculation growth medium added and incubated at a known temperature for 2-3 days. Potable =22 and 36 deg C respectively Membrane Filtration (MF) 100ml inoculation - growth medium added and incubated at a temperature between 30 35 deg C for 5 days 4187 Guideline Presentation- March 2015

6 3. What is TVC how is it measured? 4187 Guideline Presentation- March 2015 7 3. What is TVC how is it measured?

4187 Guideline Presentation- March 2015 8 4. What are Endotoxins & How are they analysed? Endotoxins are large molecules consisting of a lipid and a polysaccharide (LPS) and are found in the outer membrane of

Gram-negative bacteria Endotoxins are often released upon cell death but can be excreted by gram ve bacteria as a defence mechanism Endotoxins when introduced into human body, can cause a

fever-like reaction 4187 Guideline Presentation- March 2015 9 4. What are Endotoxins & How are they analysed? ALS currently perform Bacterial Endotoxin analysis in our Brisbane laboratory Based on clotting reaction of horseshoe crab blood

Sample is incubated with an LAL standard (Limulus Amebocyte Lysate) at a known sensitivity and the clotting time is measured and plotted on a standard curve The sample is collected in a sterile & non-pyrogenic 50mL tube HT of 24hrs currently running extended HT data TAT 5-7 days Fast TAT available (1 day) 10

5. Sampling & Transport to the Laboratory Aseptically collected flaming / ethanol Appropriate containers sterile, non-pyrogenic Correct chilling - <4 deg on receipt Holding time TVC analysed within 24hrs of collection 11 6. Guideline Report

12 7. Questions 4187 Guideline Presentation- March 2015 13

Recently Viewed Presentations

  • The Marijuana Experiment

    The Marijuana Experiment

    Number of Cultivator Licenses In accordance with rule 3796:2-1-01 of the Administrative Code, the Department may issue up to 12 Level I and 12 Level II cultivator provisional licenses before September 9, 2018. ... No more than six and six-tenths...
  • Evolution - MS. SMITH 8TH GRADE SCIENCE

    Evolution - MS. SMITH 8TH GRADE SCIENCE

    From evolution.Berkeley.edu. Maybe mention the Wallace line. Could throw in something about plate tectonics. 3. The Law of Superposition. Successive layers of rock or soil were deposited on top of one another by wind or water.
  • Students transforming Student enga gement throu gh working

    Students transforming Student enga gement throu gh working

    Engagement through partnership: students as partners in learning and teaching in higher education by Mick Healey, Abbi Flint and Kathy Harrington; HEA 2014. UEA's Code of practice for student representation. Any questions?
  • Global Coalition for Peace presents Basic Seed-Saving Workshop

    Global Coalition for Peace presents Basic Seed-Saving Workshop

    Global Coalition for Peace presents Basic Seed-Saving Workshop
  • Properties of Matter - Denton ISD

    Properties of Matter - Denton ISD

    Properties of Matter 5.5 Matter and energy. The student knows that matter has measurable physical properties and those properties determine how matter is classified, changed, and used.
  •  11

    11

    第11單元 陳峙維教授
  • Royal Commission into Institutional Responses to Child Sexual ...

    Royal Commission into Institutional Responses to Child Sexual ...

    180 Flinders Street, Melbourne VIC 3000 141 Queen Street, Brisbane QLD 4001. PO Box 84 Flinders Lane VIC 8009 PO Box 2151 Brisbane QLD 4001 (03) 8663 7490 (07) 3218 4500. You can phone . knowmore. toll-free . 1800 ....
  • Statistics - SSRIC

    Statistics - SSRIC

    Bar Chart. We'll use the GSS10A data set. Click on Graphs and then on Chart Builder. Make sure that the Gallery tab is selected and then click on Bar. Click on the top left bar chart (i.e., simple bar chart)...