Methods Plasmid construction: ePTAP,hPTAP,pL,pG2A and more . Yeast two-hybrid assays- yeast cell transformed with tagged -Tsg101and tagged -Gag or Vp40 plasmids. HA,p24,myc are monoclonal antibodies used as tags. Western-blot analysis- yeast cell , mammalian cell ,HIV-1 virions and EbVp40 virus...
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