Kuby's Immunology Complement

Kuby's Immunology Complement

Complement & Complement Fixation Hugh B. Fackrell 02/28/20 1

Content Outline Complement Components Complement Activation Biological Consequences

Complement Fixation 02/28/20 2 02/28/20 3

02/28/20 4 02/28/20 5

Complement system 02/28/20 6 Phases in the Complement Cascade Recognition

Activation Amplification Membrane Attack 02/28/20 7 Complement Cascade

02/28/20 8 Complement Activation Classical pathway Alternative pathway Membrane attack complex

02/28/20 9 Complement Components The Classical Pathway

02/28/20 10 Membrane Bound Antigens 02/28/20

11 Soluble Antigen 02/28/20 12 02/28/20

13 02/28/20 14 C4

02/28/20 15 C2b 02/28/20 16

C3 02/28/20 17 C5

02/28/20 18 C5a & C5b 02/28/20 19

C6 02/28/20 20 C7

02/28/20 21 C7 becomes hydrophobic 02/28/20 22

C8 02/28/20 23 C8 in membrane

02/28/20 24 C9 02/28/20 25

C9 pore 02/28/20 26 02/28/20

27 An Alternative Complement Pathway Part of Innate immunity Activated without Antibody 02/28/20

28 Alternate Vs Classical Paths 02/28/20 29

Regulation of Complement 02/28/20 30 02/28/20 31

Biological Consequences Cell lysis Inflammation Opsonization Viral neutralization Solubilization of immune complexes

02/28/20 32 02/28/20 33

02/28/20 34 02/28/20 35 Complement Fixation Test

Fig 18.8 02/28/20 36 Assay Test Phase

Positive serum antibodies

Negative serum no antibodies Antigen Complement No Ag-Ab complex Complement NOT

used up Antigen Complement AG-AB complex formed

Complement used up No visible difference!!! 02/28/20 37

Assay Indicator phase Positive Test phase

No complement Indicator system Complement Sheep RBCs

Anti sheep RBCs Result NO LYSIS of RBCs CLOUDY 02/28/20

Negative test phase Indicator System Sheep RBCs Anti Sheep RBCs Result

LYSIS of RBCs CLEAR 38 CF: Advantages Reagents easily available Semi quantitative or quantitative Wide application

Extremely sensitive 02/28/20 39 CF: Disadvantages Indirect Test

Need adequate controls Reagents unstable Quantitation time consuming Cannot be automated 02/28/20 40

DONE!!! 02/28/20 41

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