Paper Chromatography - Pepperdine University

Paper Chromatography - Pepperdine University

Paper Chromatography Identifying the components of a mixture Chromatography Chromatography is a method for separating mixtures into their components based on physical and/or chemical properties of the components. Developed around 1903 by Russian Mikhail Semenovich Tswett in which he

separated plant pigments on diatomaceous earth with alcohol Uses for Chromatography Chromatography can be used to: Qualitatively analyze the components of a mixture Qualitatively identify the components of a mixture using known compounds Quantitatively determine the amount of a component in a mixture using standard

samples Purify individual components by separating them from the other compounds in a mixture The Basics Mixture is placed on stationary phase Mobile phase passes over the stationary phase Mobile phase dissolves the components

Mobile phase carries the individual components a certain distance through the stationary phase, depending on their attraction to both of the phases Solvent Front Chromatographed Spot Rf ratio of spot distance to front Putsolvent

the spotted paper distance tank in a developing Origin line Solvent Principles of Paper Chromatography Capillary Action the movement of liquid within the spaces of a porous material due to the forces of

adhesion, cohesion, and surface tension. The liquid is able to move up the filter paper because its attraction to itself is stronger than the force of gravity. Solubility the degree to which a material (solute) dissolves into a solvent. Solutes dissolve into solvents that have similar properties. This allows different solutes to be separated by different combinations of solvents. Separation of components depends on both their solubility in the mobile phase and their differential affinity to the mobile phase and the stationary phase.

Investigation: What Dyes are in the M&Ms and Food Colors? Preparing the Chromatography Paper Obtain an 7 x 8 cm of chromatography paper With a pencil (why?), draw an origin line 1 cm above the bottom edge of the strip. Near the top, ID the chromatogram Mark the origin line with a lane mark each cm. No lane

can be closer than about 1 cm from the edge Preparing the Chromatography Paper ID each lane and record what each lane is spotted with Place a spot from each solution on the origin More than one strip may be required to analyze all of the solutions

Developing the Chromatograms Add chromatographic solvent to a sufficiently large beaker to a depth of less than 1 cm Place the spotted paper in the beaker carefully Ensure the solution does not go above the origin line Develop the chromatogram until the solvent front is about 2 cm from the top of the paper

Developing the Chromatograms Remove the chromatogram and place on a paper towel, trace the solvent front with pencil, and let the chromatogram dry in the oven for a few minutes. After the chromatogram is dry, calculate the retention factors, Rf, and tabulate the values Determine the dye content of each of the unknowns

Considerations Small spots are generally better but harder to see after developing UV lamps, if available can (sun)burn your eyes but UV is stopped by the plastic of your goggles UV lamps are low power but extended exposure can cause a mild (sun)burn Dont eat the M&Ms theyve been in the lab awhile

Writing Procedure Proposals Clearly state your experimental methods and how those procedures will yield solutions to the problems addressed in the investigation. State what data you plan to collect and how you will analyze the data. Include proposed data tables. State all the materials you plan to use including, if possible, concentrations and quantities. Describe the safety hazards associated with your analysis and appropriate precautions you will take to avoid personal injury.

Each team member must sign and date the procedure proposal before turning it in.

Recently Viewed Presentations

  • Decreasing Behavior with punishment

    Decreasing Behavior with punishment

    Definitions Punisher Event that, when presented immediately after a behavior, causes that behavior to decrease in frequency Synonyms Aversive stimuli Aversives Principle of Punishment If in a certain situation a person engages in a behavior that is followed immediately by...
  • Roman Britain With gratitude to Sr. Therese Dougherty

    Roman Britain With gratitude to Sr. Therese Dougherty

    Slide 14 Slide 15 Boudicca's Rebellion 60 A.D. Coloniae, Municipia, Civitates Plan of Corinium Dobunnorum Slide 19 Artist's conception of Theatre at Verulamium Roman Theatre discovered in Kent Colchester Vase Verulamium Town House Slide 24 Verulamium Museum Oceanus Mosaic Verulamium...
  • BHP Steel - BlueScope

    BHP Steel - BlueScope

    A skills test (literacy, numeracy, mechanical reasoning) to further screen applicants. An interview panel or series of interviews where 1-2 interviewers will ask questions from different stand points and then agree on the successful candidate. ... Tips for Making a...
  • Diapositive 1 - High-Tech

    Diapositive 1 - High-Tech

    - Stabilité et bornitude entrée/sortie : Un système linéaire est stable si et seulement si à tout signal borné en entrée, correspond un signal borné en sortie, c.à.d. : u borné y borné - Stabilité et pôles d'un système linéaire...
  • Radiation Protection in Diagnostic Radiology

    Radiation Protection in Diagnostic Radiology

    RADIATION PROTECTION IN DIAGNOSTIC AND INTERVENTIONAL RADIOLOGY L 01. Overview of Radiation Protection in Diagnostic & Interventional Radiology Motivation for the Course * These changes since the early 1990s provide a backdrop to the current use of radiation in medical...
  • CAP6135: Malware and Software Vulnerability Analysis Botnets Cliff

    CAP6135: Malware and Software Vulnerability Analysis Botnets Cliff

    Researchers in Gtech monitored thousands of botnets * Spreading Problem Spreading mechanism is a leading cause of background noise Port 445, 135, 139, 137 accounted for 80% of traffic captured by German Honeynet Project Other ports 2745 - bagle backdoor...
  • CSR in Hong Kong: A Political Sociology Perspective

    CSR in Hong Kong: A Political Sociology Perspective

    Data on Corporate Social Responsibility in HK CSR Survey of Hang Seng Index Constituent Companies 2009 - Oxfam Hong Kong Caring Company Scheme - Hong Kong Council of Social Service Survey documents the CSR policies and initiatives of the 42...
  • JavaScript: How To?

    JavaScript: How To?

    The Assignment Statement <variable> <assignment symbol> <expression>; <variable> is any declared variable in the program <assignment symbol> is the language's notation for the assignment operation <expression> is a kind of formula telling the computer how to compute the new value....