The world leader in serving science Rhombix Solutions
The world leader in serving science Rhombix Solutions for Automated Crystallization 2007 RAMC Rhombix Product Line supporting the crystallization workflow Semi-automated imaging Rhombix Software Suite MaX Workcell 2 Automated imaging and storage Instrument-Centric to Data-Centric Automation Liquid handler Dispense screens into wells Nanodispenser Drop-making Xtallization
Database Experiment design and analysis Imaging and Storage Result: Isolated islands of automation and data stores -- No data connection 3 New Product New MaX Workcell Plate preparation 4 MaX Workcell Features & Benefits All-inclusive product pre-configured for quick installation Seamless Workflow Tube-to-plate transfer Plate-to-plate transfer Custom screen building (gradients, additives, etc) Nano Drop placement Sealing Multiple experiment design and submission
Full set of SW tools incorporating experimental data and automation control Small footprint 5 Imaging Modes Brightfield White LED light source Eliminates heating of plates Darkfield Changes the angle of incidence mitigating shadow regions associated with Brightfield Assists visualization of crystals Polarization True polarization Variable angle transmitter and analyzer Exploits birefringent property of crystals
6 Lighting and treatment options Brightfield Darkfield Variable Angle Polarization Extinction Extinction + 45 7 Extinction + 90 Imaging Features Drop-finding X-focus (extended depth of focus) Others: autofocus, autoexposure, sharpening 8 Rhombix Software Suite Supporting the Crystallization Workflow Protein Crystallization Software Packages Reagent Manager
5.1 pH 5.3 5.9 6.1 Queries Query Summary 48 matching records f ound, 8 records selected V iew Results... A nnotate... 4 Hampton II Hampton 2 Srceo I Hampton UserUserdefined defined values
values 6.2 Sav e Screen Main 10 Viewing and Annotation -- Clarix interface for viewing and annotating images brings in information from database (e.g. well contents & drop details) provides feedback to imaging link to Screen Design for optimization screens link to data-mining link to scheduling 11 Report Tool Report across users and experiments Export to .xls, .pdf, HTML, xml
formats One means of data sharing between sites (export at one site, import at another site) 12 Data Mining Visual Query Tool Graphical data-mining SW for general queries and experiment optimization planning Links to Viewing application for display of image results 13 Screen Designer Graphical interface for designing screens Allows concentration and pH gradients Gradients across grids, rows, columns Patterns of start/step, start/end, center/step 14 Import Utility
Imports chemicals, screening solution formulations, screens, and experiments Pair with report tool for data sharing 15 More Insight and Less Mystery in Crystallization Tools for easy data retrieval and viewing Tools for easy data entry Drop to Crystal Workflow 16
Amphibians Review #2 #1 is sends messages to this part of the brain This part of the digestive system is the _____ Green = _____ This holding on to the female so eggs and sperm are released together is called...
21st Century Curriculum ... and then the notes related to the problems with this design on p.178 in your UbD workbook. This is an example of a check for validity that reveals problems. ... Do not use criterion-based performance lists...
Outcome Harvesting's utilization focus requires that primary intended users participate in decision-making throughout the harvest. Evaluator. assumes responsibility of . convening users . to participate in decision-making throughout the harvest, beginning with the design workshop. Dilemma. Resolution. E. Manage development...
axial. portion (head, neck, and trunk) and an . appendicular. portion (upper and lower limbs) The axial portion contains several cavities that house body organs: - Cranial cavity - brain - Vertebral canal - spinal cord.