The world leader in serving science Rhombix Solutions

The world leader in serving science Rhombix Solutions

The world leader in serving science Rhombix Solutions for Automated Crystallization 2007 RAMC Rhombix Product Line supporting the crystallization workflow Semi-automated imaging Rhombix Software Suite MaX Workcell 2 Automated imaging and storage Instrument-Centric to Data-Centric Automation Liquid handler Dispense screens into wells Nanodispenser Drop-making Xtallization

Database Experiment design and analysis Imaging and Storage Result: Isolated islands of automation and data stores -- No data connection 3 New Product New MaX Workcell Plate preparation 4 MaX Workcell Features & Benefits All-inclusive product pre-configured for quick installation Seamless Workflow Tube-to-plate transfer Plate-to-plate transfer Custom screen building (gradients, additives, etc) Nano Drop placement Sealing Multiple experiment design and submission

Full set of SW tools incorporating experimental data and automation control Small footprint 5 Imaging Modes Brightfield White LED light source Eliminates heating of plates Darkfield Changes the angle of incidence mitigating shadow regions associated with Brightfield Assists visualization of crystals Polarization True polarization Variable angle transmitter and analyzer Exploits birefringent property of crystals

6 Lighting and treatment options Brightfield Darkfield Variable Angle Polarization Extinction Extinction + 45 7 Extinction + 90 Imaging Features Drop-finding X-focus (extended depth of focus) Others: autofocus, autoexposure, sharpening 8 Rhombix Software Suite Supporting the Crystallization Workflow Protein Crystallization Software Packages Reagent Manager

Screen Designer Experiment Manager Schedule Editor Barcode Manager Deck Manager Experiment Launcher Screen Monitor Vision Monitor Equipment Monitor Visual Query Tool Image Viewing and Annotation (Clarix) Report Tool Experiment Design Experiment Execution Results Analysis and Scoring Data Mining

Optimization Visual Query Tool Image Viewing and Annotation (Clarix) Screen Designer 9 Knowledge Rhombix Software Crystal Plate Query File Tools Help my Query ? Fourteen Software Applications Built into an Cohesive Framework 3D-Bar SearchItems Rich TargetID

T RIS pH7.5 (20mM) Spanning: Reagent Management to Data-Mining Results DB Rysosome24 (0.1%) Created 5/1/01 -8/12/02 Precipitant pH Greg Date Ethelyne Glycol (10mM) pH = 5.2- 6.4 Additive User

Reagent Temperature Clarity pH Method Screen lysozome 4.2 Vapordiffusion Hampton II lysozome 4.2 Vapordiffusion Hampton II lysozome

4.3 Vapordiffusion Hampton I lysozome 4.2 Vapordiffusion Hampton I lysozome 5.1 Vapordiffusion Hampton I murB-1 6.2 Hanging drop Hampton II

murB-1 6.2 Hanging drop Hampton II murB-1 5.9 Microbatch Hampton I murB-1 4.2 Microbatch Hampton I murB-1 4.4 Hanging drop

Hampton thrombin 4.3 Vapordiffusion Hampton II thrombin 5.1 Vapordiffusion Hampton II lysozome 5.1 Hanging drop Hampton II 9 8

Occurrence Target ID Screen 7 6 5 4 2 3 3 2 1 4 0 4.2 4.3 4.4 4.6

5.1 pH 5.3 5.9 6.1 Queries Query Summary 48 matching records f ound, 8 records selected V iew Results... A nnotate... 4 Hampton II Hampton 2 Srceo I Hampton UserUserdefined defined values

values 6.2 Sav e Screen Main 10 Viewing and Annotation -- Clarix interface for viewing and annotating images brings in information from database (e.g. well contents & drop details) provides feedback to imaging link to Screen Design for optimization screens link to data-mining link to scheduling 11 Report Tool Report across users and experiments Export to .xls, .pdf, HTML, xml

formats One means of data sharing between sites (export at one site, import at another site) 12 Data Mining Visual Query Tool Graphical data-mining SW for general queries and experiment optimization planning Links to Viewing application for display of image results 13 Screen Designer Graphical interface for designing screens Allows concentration and pH gradients Gradients across grids, rows, columns Patterns of start/step, start/end, center/step 14 Import Utility

Imports chemicals, screening solution formulations, screens, and experiments Pair with report tool for data sharing 15 More Insight and Less Mystery in Crystallization Tools for easy data retrieval and viewing Tools for easy data entry Drop to Crystal Workflow 16

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