Section II Artificial Insemination Technique Deposit semen at

Section II Artificial Insemination Technique  Deposit semen at

Section II Artificial Insemination Technique

Deposit semen at the right place

90% lost due to retrograde flow (Mitchell et al. 1985)

Can horn breeding reduce retrograde loss and help to

improve fertility??? Horn vs. body deposition

Positive effect Zavos et al., 1985 Lopez-Gatius and

Camon-Urgel, 1988 Senger et al., 1988 Seidel et al., 1999 (1/5)

Pursley et al., 2004 Drisken et al., 2004

No effect Elliott, 1944 Brandt et al., 1946

Knight et al., 1951 Salisbury and VanDemark, 1951

Olds et al., 1953 Macpherson, 1968 Williams et al., 1988

Momont et al., 1989 McKenna et al., 1990 Lang-Ree, 1992

Grieger et al., 1998 Seidel et al., 1999 (4/5) Kurykin et al., 2003

Andersson et al., 2004 Verberckmoes et al, 2004 Drisken et al., 2004

Negative effect Graves et al., 1991

(P < 0.10) Effects of horn breeding (bilateral) with low

cell number doses (1 - 3 x 106) of sexed semen on AI conception rate 80

Body

Horn Percent

*

24 14 40

n=

48 50 52 48

86 86

29 32 0

1 2

3

Study (Adapted from Seidel et al., 1999, Therio. 52:1407)

4 5

Ipsilateral comparable to contralateral after deep unilateral semen deposition

Hawk and Tanabe, 1986 Larsson, 1986 Pallares et al.,1986

Momont et al., 1989 Lpez-Gatius, 1996 Seidel et al., 1997

Get semen through cervix!!!

Summary Be gentle dont use too much force Two step process

Get gun to the cervix Pass cervix over the gun

Deposit semen in uterine body Take your time Relax

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