haplotype host of origin resistance gene Couch et

haplotype host of origin resistance gene Couch et

haplotype host of origin resistance gene Couch et al. 2005 Magnaporthe oryzae Signaling on the leaf surface positive regulation negative regulation stage of development signal

receptor + adenyl cyclase MAPK MAPK transcription factor

X heterotrimeric G protein dissociation protein kinase spore tip mucilage Howard and Valent 1996 Cosegregation of the appressorium deficiency phenotype

with the mpg1::Hph deletion allele. WT mpg1- hygS hygR hygS MPG1 directs formation of the hydrophobic rodlet layer of conidia.

WT mpg1- hygR WT mpg1MPG1+ MPG1+ Reintroduction of MPG1 restores pathogenicity, appressorium development and cell surface hydrophobicity

mpg1-;hygR MPG1-;hygS Importance of melanized appressoria Howard, R chitin WT albino

melanin appressorium pore Howard and Valent 1996 Penetration and invasion penetration site Dean et al, 2005

melanin biosynthesis Howard and Valent 1996 Collapse high concentration of non-permeable solute conidium germ tube appressorium sonicated cells

Howard and Valent 1996 Appressoria build turgor during incubation Howard et al., 1991 The transcription factor MST1 is important for penetration peg formation homeodomain WT

Zn finger mutants Park et al. 2004 The transcription factor MST1 is important for penetration peg formation mst1WT appressoria can form cant penetrate

no peg From Park et al 2004 Fungal avirulence genes and others AVR-Pi-ta Lauge and de Wit 1998 four Avr genes; Avr2, Avr4, Avr4E

and Avr9 four extracellular protein (Ecp) genes; Ecp1, Ecp2, Ecp4 and Ecp5). M. oryzae Pi-ta / AVR-Pi-ta Fig. 2. Genotype-specific HR in rice seedlings induced by M.grisea carrying AVR-Pita. Sparse HR flecking is seen in Pi-tacontaining rice seedlings (A) Yashiro-mochi and (B) YT14, as expected. In contrast, typical symptoms of rice blast disease are seen in susceptible rice seedlings (C) Nipponbare and (D) YT16. Representative leaves are shown from rice seedlings germinated in plant nutrient medium and infected with avirulent M.grisea strain 4360-R-62 (see Materials and methods for details). Shown at 4 days after inoculation Jia et al. 2000 Pi-ta

YM YT14 resistant pi-ta Ni YT16 susceptible Pi-ta dependent resistance response Genotype specific response Intracellular (metalloprotease) direct interaction with Pi-ta Fig. 3. AVR-Pita176 is an elicitor. (A) AVR-Pita polypeptides tested in the transient assay. The white

region indicates the putative secretory signal sequence, the gray region indicates the putative pro-protein domain and the hatched region indicates the putative protease motif. The black region indicates the putative mature protein. The number of amino acids missing from the N-terminus is indicated. GUS activity is indicated by +, whereas decreased GUS activity is indicated by . (B) Representative rice seedlings showing GUS activity. Two-leaf Pi-ta (Yashiro-mochi and YT14) and pi-ta (Nipponbare and YT16) seedlings were co-bombarded with 35S/Adh1-6::AVR-Pita176 and 35S::uidA. Leaves were assayed histochemically for GUS activity and cleared in 70% ethanol to visualize GUS staining. (C) RNA gel blot analysis of AVR-Pita expression in the transient assay. YT14 (Pi-ta) and YT16 (pi-ta) were cobombarded with the 35S/Adh1-6::AVR-Pita176 and 35S::uidA plasmids. Leaf tissue was harvested 2 days after bombardment. Poly(A)+ mRNA was then extracted, blotted to Hybond-N and hybridized with a radiolabeled AVR-Pita176 probe. The AVR-Pita transcript is indicated. Similar loading was verified before blotting by visualizing mRNA in the gel stained with ethidium bromide. Jia et al. 2000 Magnaporthe oryzae

is also a root pathogen hydrophobic surface roots ac, GFP-tagged M. grisea (Guy11) forms classical appressoria (AP) on a hydrophobic surface (a) and simple hyphopodia (HY) and infection pegs (IP) on rice roots (cultivar CO39) (b, c). dk, Guy11infected barley (d, e, j, k) and rice (fi) roots stained with chlorazole black E showing: dark runner hyphae (RH) and simple hyphopodia (d, e); bulbous infection hyphae invading epidermal cells (f); microsclerotia (previously reported in culture). Scale bar, 25 m. Sesma and Osbourn Sesma and Osbourn

Pigment and cAMP are not required albino cAMP- albino a, Roots of barley seedlings (cultivar Golden Promise) that have been mockinoculated or infected with the M. grisea wild type (WT) or mutant (mel, cpkA) strains. b, Formation of hyphopodia-like structures (HY) and invasive growth within epidermal cells

during the early stages of infection. Scale bars, 25 m (b), 40 m (c). cAMPSesma and Osbourn M. oryzae can move systemically from roots to leaves Roots of barley seedlings (cultivar Golden Promise) that have been mock-inoculated or infected with the M. grisea wild type (WT) strain.. c, M. grisea penetrates the stele. Confocal imaging of radial and longitudinal sections of a three-week-old rice seedling (cultivar Nipponbare) infected with GFP-tagged M. grisea (strain

Guy11). Scale bars, 25 m (b), 40 m (c). Sesma and Osbourn M. oryzae can move systemically from roots to leaves Pi-CO39(t)mediated specific disease resistance operates in

rice roots ac, Four-week-old root-infected rice seedlings (cultivar Nipponbare) showing disease symptoms on the leaf (upper box) and collar (lower box) (a). Disease symptoms on the collar (b) and stem (c) with confocal images showing GFPexpressing M. grisea Guy11 in the diseased areas and also in the vascular tissue of the leaf and stem. df, Pi-CO39(t)-mediated specific disease resistance operates in rice roots. Confocal microscopy of compatible (d, e) and incompatible (f) interactions. Cultivar, cv. Scale bar, 40 m. Sesma and Osbourn

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