300 Gastro 2001;121:195 Gene Testing for MSH2/MLH1 509 Finnish CRC pts 5/10 Founder mutation 63 MSI 7/10 Amsterdam Criteria All either young, had fam hx, or previous CA
10 (2%) MMR mutations Aaltonen, NEJM 1998;38:1481 Predictive Model for MMR Gene Testing 184 Kindreds: 26% w/ MMR mutations 1) Mean age at diagnosis of affecteds 2) At least 1 member w/ Endometrial CA 3) Amsterdam Criteria Wijnen, NEJM 1998;339:511 Predictive Model for MMR Gene Testing Logistic Model
Bethesda Criteria and MMR Mutation N=125, high risk, Frankfurt, GE + BC - BC Total N 58 (46%)
67 (54%) 125 MSI 17 (29%) 5 (7.5%) 22 (18%)
0 (0%) 11 (9%) MMR Mutation 11 (65%) B1 - B4 46 (79%) Raedle, Ann Int Med 2001;135:566 Bethesda vs. Amsterdam MMR Mutation
MSI status Criteria to predict MSI Sens Spec Amsterdam 6/6 27
94 Amsterdam II 8/10 46 90 Bethesda
11/17 77 60 Raedle, Ann Int Med 2001;135:566 Cost Effectiveness of MSI Decision tree using MSI (Bethesda guidelines) and MMR mutations 90% CI for cost-effectiveness of screening patients with cancer & relatives:
gained $4,874 - 21,576 / life year Sensitivity analysis - prevalence HNPCC mutation #1 factor Ramsey, Ann Int Med 2001;135:577 Click for larger picture Mutations in HNPCC Kindreds 32 Kindreds (N=38) in Buffalo and Vermont
Amsterdam Criteria Incidence of Mutations MSH2/MLH1: 25% Conclusion: Molecular basis unknown for many subjects Weber, Cancer Res 1997;57:3798 Effectiveness of Screening in HNPCC 252 subjects, 22 Families (119 Control, 133 screen) Colon q3yrs, 1984, 15 yr F/U Not randomized - declined participation Screen
CRC Mutation + Deaths to CRC 8 (6%) 18% 0 Control OR 19 (16%) 41% 8%
.4 .4 P .01 .02 <.001 Jarvinen, Gastro 2000;118 Click for larger picture Risk of Metachronous CRC
50 40 30 Population MMR Mutation 20 10 0 10 y
15 y Colonoscopy in High Risk Individuals 31 HNPCC Families - 232 Individuals 86 (38.6%) underwent colonos-compared to controls Case CA Adenomas TV/V (#) Ad Diam HGD (#)
5 29 11 9.1 9 Control 1 11 1 5.8 3
P .03 .02 Ponz de Leon, CEBP 1998;7:639 Center for Families at Risk for CRC Jan 98 - June 00 Goal: To develop a registry of high risk families To assemble blood/DNA for research Recruitment: Physician referral, Media, UPCI CA Registry High Risk Definition: Young onset, FDR young onset, Multiple cancers
Dead 106 23 Not Interested Enrolled 33 Unavailable Young onset cancers - <45, 45-55 High Risk Patients
70 Probands - Complete data, exclude FAP 67.1% High Risk 23 Young Onset (<55) 9 Multiple CAs 15 Young and Multiple (8 Amsterdam Criteria) Problems With Center Lab Support Integrated Recruitment Coordinated Approach With Other Cancers Gene Testing
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